oral administration of vitamin c, cimetidine and famotidine on micronuclei induced by low dose radiation in mouse bone marrow cells
نویسندگان
چکیده
background: in many studies, chemicals and natural materials were tested to reduce the harmful effects of radiation. it is known that famotidine and vitamin c reduce dna damage. objective: the aim of this study was to evaluate the radioprotective effect of vitamin c, cimetidine and famotidine on gamma-radiation-induced damage on mouse bone marrow. methods: six-to-seven week male nmri mice (28 g ±3) were randomly divided into fourteen groups: control, 2gy irradiation, six group drugs without irradition (famotidine, cimetidine, vitaminc, fam-cim, fam-vit, cim-vit), six groups received drugs and 2gy radiation with a 60co |γ|-ray source at room temperature 22 ± 2 °c. the mice were killed 48 hours after irradiation by cervical dislocation. slides were prepared from bone marrow cells and stained in may-granwald and giemsa. finally, the cells were counted with microscope, frequencies of polychromatic erythrocyte (pce), normochoromatic erythrocyte (nce) and their micronuclated cell were recorded. pce / pce + nce were calculated. results: there were significant differences of mnpce/1000pce, mnnce/1000nce and pce/pce+nce among different groups with similar radiation doses (p≤0.01). moreover, there were significant differences of mnpce/1000pce and pce/pce+nce among different doses of radiation (p≤0.01). while considering mnnce/1000nce, there were no significant differences among silimar groups with radiation dose (p˃0.05). conclusion: oral administration of famotidine, vitamin c and cimetidine demonstrate reliable and similar radioprotective effects. additionally, the protective effect of single use of these drugs was similar to the combination form. thus, the oral use of combination, 48 hours after irradiation cannot induce more radioprotective effect.
منابع مشابه
Oral Administration of Vitamin C, Cimetidine and Famotidine on Micronuclei Induced by Low Dose Radiation in Mouse Bone Marrow Cells
 Background: In many studies, chemicals and natural materials were tested to reduce the harmful effects of radiation. It is known that Famotidine and vitamin C reduce DNA damage.Objective: The aim of this study was to evaluate the radioprotective effect of vitamin C, Cimetidine and Famotidine on gamma-radiation-induced damage on mouse bone marrow. Methods: Six-to-seven week male NMRI mice (28...
متن کاملOral Administration of Vitamin C, Cimetidine and Famotidine on Micronuclei Induced by Low Dose Radiation in Mouse Bone Marrow Cells
BACKGROUND In many studies, chemicals and natural materials were tested to reduce the harmful effects of radiation. It is known that Famotidine and vitamin C reduce DNA damage. OBJECTIVE The aim of this study was to evaluate the radioprotective effect of vitamin C, Cimetidine and Famotidine on gamma-radiation-induced damage on mouse bone marrow. METHODS Six-to-seven week male NMRI mice (28 ...
متن کاملeffects of famotidine and vitamin c on low dose radiation-induced micronuclei in mice bone marrow cells
the radioprotective effects of vitamin c and famotidine were investigated using the micronucleus test for anticlastogenic and cell proliferation activity. various doses of vitamin c and famotidine were administered intraperitoneally 2 h before 2gy gamma irradiation to nmri adult male mice. frequency of micronuclei in 1000 pces (mnpces) were scored for each sample. cell proliferation ratio (pce/...
متن کاملEffects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and Its Definite Dose Reduction Factor
Introduction: Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation. In the present study,the effects of arbutin were investigated by using the micronucleus test for anti- clastog...
متن کاملEffects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor
Background: Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation.In the present study, the effects of arbutin were investigated by using the micronucleus test for anti-clastogenic activity, to cal...
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Background: Radioprotective effect of famotidine was previously shown on radiation induced micronuclei and chromosomal aberrations in human peripheral lymphocytes and mouse bone marrow cells however, its radioprotective property has never been studied in mouse spermatogenesis. It was also shown that vitamin C as an antioxidant also exert its radioprotective effect on many biological s...
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عنوان ژورنال:
journal of biomedical physics and engineeringجلد ۲۰۱۲، شماره ۱۲، صفحات ۰-۰
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